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KMID : 0363320100310020273
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Journal of Korean Oriental Internal Medicine
2010 Volume.31 No. 2 p.273 ~ p.289
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Studies on the Anti-cancer Effect and the Mechanism of Apoptosis by Baekduong-tang in Human Colon Cancer Cell Line HCT-116
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Kim Jong-Uk
Moon Goo
Park Chan-Hee
Lee Jeong-Han
Ji Hye-min
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Abstract
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Objectives : To investigate the anti-cancer effect of Baekduong-tang(BDOT) against cancer cells, the signaling pathway of apoptosis was explored in human colon cancer cells.
Materials and Methods : Human colon cancer cell lines, including HT-29 and HCT-116 cells, were used. Cell viability was measured by MTT assay. Apoptosis was determined by DAPI nuclei staining and flow cytometry in HCT-116 cells treated with 0.25 mg/ Baekduong-tang for 48 hrs.
Results : Baekduong-tang induced the apoptosis of p53 positive HCT-116 cells with G2/M phase arrest. Treatment with Baekduong-tang led to increased expression and phosphorylation of p53 and decreased expression of CDK2 and CDK6 in HCT-116 cells. It also activated caspase-3 through caspase-10 and caspase-9 activation. Finally, Baekduong-tang induced production , superoxide anion () and NO and modulated proteins expression including SOD, NOS, Bax and Bcl-2.
Conclusions : These results indicate Baekduong-tang induces apoptotic death of HCT-116 cells through G2/M phase arrest and disturbance of intracellular redox status in a p53-dependent manner.
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KEYWORD
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Baekduong-tang(BDOT), colon cancer, HCT-116 cells, apoptosis
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